博茨瓦纳HIV相关隐球菌脑膜炎患者的脑脊液和血浆中HIV-1C env和...

来自 : www.x-mol.com/paper/1336022073 发布时间：2021-03-25

HIV-1 compartmentalization in reservoir sites remains a barrier to complete HIV eradication. It is unclear whether there is variation in HIV-1 env and gag between cerebrospinal fluid (CSF) and plasma of individuals with HIV-associated cryptococcal meningitis (CM). We compared HIV-1 env characteristics and the gag cytotoxic T-lymphocyte (CTL) escape mutations from CSF and plasma samples. Employing population-based Sanger sequencing, we sequenced HIV-1 env from CSF of 25 patients and plasma of 26 patients. For gag, 15 CSF and 21 plasma samples were successfully sequenced. Of these, 18 and 9 were paired env and gag CSF/plasma samples, respectively. There was no statistically significant difference in the proportion of CCR5-using strains in the CSF and plasma, (p = 0.50). Discordant CSF/plasma virus co-receptor use was found in 2/18 pairs (11.1%). The polymorphisms in the HIV-1 V3 loop were concordant between the two compartments. From the HIV-1 gag sequences, three pairs had discordant CTL escape mutations in three different epitopes of the nine analyzed. These findings suggest little variation in the HIV-1 env between plasma and CSF and that the CCR5-using strains predominate in both compartments. HIV-1 gag CTL escape mutations also displayed little variation in CSF and plasma suggesting similar CTL selective pressure.
2020年12月7日Moraka; Kwana Lechiile; Tshepo B. Leeme; David S. Lawrence; Rosemary Musonda; Ishmael Kasvosve; Thomas S. Harrison; Joseph N. Jarvis; Simani...博茨瓦纳HIV相关隐球菌脑膜炎患者的脑脊液和血浆中HIV-1C env和gag的变化; Nametso Kelentse, Sikhulile Moyo, Mompati L. Mogwele, Doreen Ditshwanelo, Baitshepi Mokaleng, Natasha O. Moraka, Kwana Lechiile, Tshepo B. Leeme, David S. Lawrence, Rosemary Musonda, Ishmael Kasvosve, Thomas S. Harrison, Joseph N. Jarvis, Simani Gaseitsiwe; Viruses; Viruses; X-MOL在水库所在地的HIV-1区划仍然是彻底根除HIV的障碍。目前尚不清楚HIV相关隐球菌性脑膜炎（CM）患者的脑脊液（CSF）与血浆之间HIV-1 env和gag是否存在差异。我们比较了HIV-1 env特征和来自脑脊液和血浆样本的gag细胞毒性T淋巴细胞（CTL）逃逸突变。采用基于人群的Sanger测序，我们对25名患者的CSF和26名患者的血浆中的HIV-1 env进行了测序。对于gag，成功测序了15个CSF和21个血浆样品。其中18和9配对为env和gagCSF /血浆样品。在CSF和血浆中使用CCR5的菌株比例没有统计学上的显着差异（p = 0.50）。在2/18对中发现使用不一致的CSF /血浆病毒共受体（11.1％）。HIV-1 V3环中的多态性在两个区室之间是一致的。从HIV-1 gag序列中，三对在分析的九个中的三个不同表位中具有不一致的CTL逃逸突变。这些发现表明血浆和脑脊液之间HIV-1 env几乎没有变化，并且使用CCR5的菌株在两个区室中都占主导地位。HIV-1 gag CTL逃逸突变在CSF和血浆中也几乎没有变化，表明CTL选择压力相似。博茨瓦纳HIV相关隐球菌脑膜炎患者的脑脊液和血浆中HIV